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Size (kb): 7.0000000000000000

Vector: YIp366R (plasmid)

Promoters: Promoter none

Construction: YEp366R (ATCC 37740), YIp351

Marker(s):LEU2,ampR

Construct size (kb): 7.0

Features: insert detection: lacZ
marker(s): ampR, LEU2
promoter: none
replicon: pMB1

YI-type (integrating) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-1; SphI-2; PstI-2; SalI-1; XbaI-1; BamHI-1; SmaI-2; KpnI-2; SacI-2; EcoRI-1. The EcoRI, KpnI, and SacI sites are not unique.

Restriction digests of the clone give the following sizes (kb): HindIII-7.8; BamHI-7.8; PstI-7.8.

One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37758 - 37760) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5'AAG CTT GCA TGC CTG CAG GTC GAC TCT AGA GGA TCC CCG GGT ACC GAG CTC GAA TTC CCA GCT TGC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C