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pha2m1 [phigf4]
pha2m1 [phigf4]
規格:
貨期:
編號:B244518
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pha2m1 [phigf4]
商品貨號 B244518
Designations pha2m1 [phigf4]
Species Homo sapiens, human
Vector
Construct size (kb): 5.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: adult liver
Gene product: alpha-2-macroglobulin [A2M]
Alleles: B2, C2, E1, A2, B1, B1, C1, D1, D2, E2, F1, F2, A1, B2, E1, E2
Insert Size (kb) 2.100
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8, 1.4, 0.66; EcoRI--5.9; ClaI--4.4, 1.48; AvaI--3.3, 2.6; BamHI--5.9.
The insert encodes amino acids 809-1451 of the alpha-2-microglobulin gene.
Codominant segregation of the BglII polymorphism demonstrated in 2 families.
Codominant segregation of the PvuII polymorphism demonstrated in 5 families.
Codominant segregation of the HinfI polymorphism demonstrated in 5 families (35 individuals).
Codominant segregation of the PvuII polymorphism demonstrated in 2 families (20 individuals). HaeIII, HinfI and PuvII RFLPs are independent mutations.
Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, HincII, RsaI, HindIII, MspI, PstI.
Enzyme(s) not detecting polymorphism: AvaII, BamHI, EcoRV, StuI, AluI, NciI, MspI, PstI.
Enzyme(s) not detecting polymorphism: HindIII, KpnI, MspI, PstI, SacI, ScaI, XbaI, XmnI.
References

Bell GI, et al. Human alpha2-macroglobulin gene is located on chromosome 12. Somatic Cell Mol. Genet. 11: 285-289, 1985. PubMed: 2408344

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

Scambler PJ, et al. Two RFLPs for human alpha-2 macroglobulin (A2M). Nucleic Acids Res. 13: 6787, 1985. PubMed: 2414729

Marynen P, et al. Three RFLP's associated with the human alpha2-macroglobulin gene (A2M). Nucleic Acids Res. 13: 8287, 1985. PubMed: 2415929

Barbara Weiffenbach, personal communication

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