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pUR278-183K

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貨期:

編號:B243921

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱	pUR278-183K
商品貨號	B243921
Designations	pUR278-183K
Species	Tobacco mosaic virus
Depositors	WO Dawson
Applications	produces protein 183K protein
Vector	Construct size (kb): 6.329999923706055
Insert	DNA: cDNA Insert lengths(kb): 1.129999995231628 Gene product: 183K protein Target Gene: 183K protein
Insert Size (kb)	1.130
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments	Restriction digests of the clone give the following sizes (kb): PstI--5.6, 0.8; EcoRI--5.6, 0.8, 0.5; HindIII--6.8; BamHI/ClaI--3.3, 2.3, 1.25; BamHI--6.8; ClaI--3.6, 3.2. This clone expresses an antigen specific for the 183K protein of tobacco mosaic virus. A fragment containing the 183K gene (nt 3572 to approx. 4700) of pTMV204 was ligated into pUR278.
Classification	Tobamovirus
References	Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986. Lehto K, et al. Time course of TMV 30K protein accumulation in intact leaves. Virology 174: 290-293, 1990. PubMed: 2294644 William O Dawson, personal communication

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