人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > EBO-pSV2-neo
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
EBO-pSV2-neo
EBO-pSV2-neo
規格:
貨期:
編號:B239996
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 EBO-pSV2-neo
商品貨號 B239996
Designations EBO-pSV2-neo
Depositors RF Margolskee
Biosafety Level 1
Vector Information
Size (kb): 13.3599996566772500
Vector: EBO-pSV2-neo (plasmid)
Promoters: Promoter SV40 late
Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph)
Marker(s):G418R,ampR,hygR
Construct size (kb): 13.35999965667725
Features: marker(s): ampR, hygR, G418R
promoter: SV40 late
replicon: pMB1, EBV oriP
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6.
pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA.
A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally.
The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 黑人玩弄出轨人妻松雪| 日本中文字幕乱码aa高清电影 | 少妇高潮大叫好爽| 精品国产欧美一区二区| 久久综合九色综合欧美狠狠| 女人扒下裤让男人桶到爽| a在线观看免费网站大全| 欧美顶级少妇作爱| 国产-第1页-浮力影院| 好男人www社区视频在线资源| 亚州中文字幕午夜福利电影| 中国极品少妇xxxxx| 都市激情 在线 亚洲 国产| 亚洲精品偷拍无码不卡av| 久久精品伊人一区二区三区| 国产精品老热丝在线观看| 无码高潮又爽又黄a片软件| 日本一上一下爱爱免费| 国精产品一区一区三区| 中文在线最新版天堂| 精品毛片乱码1区2区3区 | 少妇被躁爽到高潮无码文| 人妻有码av中文字幕久久琪| a毛看片免费观看视频| 日本高清无卡码一区二区| 欧美激情a∨在线视频播放| 曰本丰满熟妇xxxx性| 精品一区二区三区影院在线午夜| 国产综合色在线精品| 国产成人无码综合亚洲日韩| 色综合久久久久综合99| 国色天香社区在线视频 | 大香伊蕉在人线免费视频| 亚洲精品一区二区三区影院| 精品国产美女福到在线不卡| 妖精色av无码国产在线看| 亚洲国产精品一区二区制服| 国产三级av在线播放| 吃奶呻吟张开双腿做受在线播放| 国产精品99无码一区二区| 亚洲一卡一卡二新区无人区 |