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pPNP1
pPNP1
規格:
貨期:
編號:B235286
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pPNP1
商品貨號 B235286
Designations pPNP1
GenBank Number

K02574

Species Homo sapiens, human
Depositors DW Martin
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 1.417999982833862
Tissue: HeLa cell line
Gene product: nucleoside phosphorylase [NP]
Insert Size (kb) 1.418
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.7, 0.58; BglII--6.0; EcoRV--4.4, 1.55; KpnI--6.0; BamHI--4.8, 1.2.
Although there is a AATAAA sequence in the insert, is is 208 bp from the 3' end of the insert which does not contain poly(A). Thus this is probably not the authentic polyadenylation signal.
Each end of the insert contains 20-22 dC/dG bp from the cloning procedure. The single long open reading frame encodes 289 amino acids, corresponding to a molecular weight of 32.1 kDa.
References

Osborne WR, Miller AD. Design of vectors for efficient expression of human purine nucleoside phosphorylase in skin fibroblasts from enzyme-deficient humans. Proc. Natl. Acad. Sci. USA 85: 6851-6855, 1988. PubMed: 3137573

Williams SR, et al. Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization. Nucleic Acids Res. 12: 5779-5787, 1984. PubMed: 6087295

Goddard JM, et al. Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli. Proc. Natl. Acad. Sci. USA 80: 4281-4285, 1983. PubMed: 6410388

Williams SR, et al. A human purine nucleoside phosphorylase deficiency caused by a single base change. J. Biol. Chem. 262: 2332-2338, 1987. PubMed: 3029074

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