| Vector |
Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RDBP
Other gene symbols: D6S45
Genomic copy number: unique
Gene name: RD protein
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.3
Tissue: acute T cell leukemia, Jurkat cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.3
Cross references: DNA Seq. Acc.: L03411 Insert lengths(kb): 1.299999952316284 Tissue: acute T cell leukemia, Jurkat cell line Gene product: RD protein( major histocompatibility complex gene RD, major histocompatibility complex, class III, RD) [RDBP] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--2.4, 1.4, 0.64; EcoRI--2.9, 1.4; HindIII--2.9, 0.68 (doublet); PstI--2.9, 1.2, 0.24; PvuII--2.5, 1.3, 0.52. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.13; PvuII--0.29; HindIII--0.58, 1.30; BglI--0.31; SacI--1.03. Contains the complete coding sequence (nt 87-1229). |
| References |
Cheng J, et al. cDNA cloning and characterization of the protein encoded by RD, a gene located in the class III region of the human major histocompatibility complex. Biochem. J. 294: 589-593, 1993. PubMed: 8373374
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