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Size (kb): 7.0000000000000000

Vector: pE8UCAT (plasmid)

Promoters: Promoter none

Construction: pEMBL8-CAT, pUMSSB1

Marker(s):ampR

Construct size (kb): 7.0

Features: insert detection: CAT
marker(s): ampR
promoter: none
replicon: pMB1
terminator: UMS
enhancer: none

promoter-cloning vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--5.4, 1.1, 0.4; HindIII--6.6.

The presence of the terminator upstream of the cloning sites of the polylinker prevents transcription that starts at cryptic promoters in the vector sequence.

This is a promoter cloning vector suitable for studying transcriptional activity of very weakly active promoter fragments.

This was constructed by inserting a 1.025 kb SalI fragment from pUMSSB1 containing the strong transcription terminator from the mouse c-mos gene (UMS = upstream mouse sequence) into the EcoRI site of pEMBL8-CAT using EcoRI linkers.

Temperature: 37.0°C