Restriction digests of the clone give the following sizes (kb): BglII--4.7, 4.0; HindIII--6.6, 1.9; EcoRI--3.9, 2.8, 1.9. pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite orientations. The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted). Contains the complete viral genome for use in site-specific mutagenesis. Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles. |
