| Vector Information |
Size (kb): 6.042 DESCRIPTION OF VECTOR:
Intact vector size: 6.042
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI
XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS411
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, ->, 575-2290
replicon: f1, <-, 2495-2951
promoter for in vitro transcription: T7, ->, 3121-3140
MCS: KpnI...SacI, ->, 3148-3250
promoter for in vitro transcription: T3, <-, 3267-3286
insert detection: lacZ', <-, 2952-3311
promoter: lac, <-, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, <-, 4468-5328
centromere: CEN6, 5470-5586
replicon: ARS4, 5587-5988 Vector: pRS412 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS402, pRS411 Marker(s):ampR,ADE2 Construct size (kb): 6.042 Features: insert detection: lacZ'
marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--2.2, 3.8; EcoRI--6.0. ade2 phenotype produces red colonies when grown on adenine containing media. This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation. |
