| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.4, 1.5, 0.6, 0.55, 0.5, 0.35; PvuII--6.1, 0.5; HindIII--6.6; BglI--1.8, 1.5, 1.2, 0.6, 0.35, 0.3 (doublet), 0.2. The insert contains the following restriction sites (nt from the 5' end): PstI--519, 528, 864, 1470, 1549; PvuII--859, 1370; StuI--416. Constructed by ligating a 376 bp StuI/ApaI fragment of the genomic clone cbmis15 containing 10 bp of 5' flanking sequence, 121 bp of 5' untranslated region, and 245 bp of the coding region (nt 80-456 of the reported sequence), to a 1666 bp ApaI/HindIII fragment of the cDNA clone pX21 containing the rest of the coding sequence and the 3' untranslated region. pBG311 is an animal cell expression vector constructed from pAT153 and the SV40 early promoter, the t splice signal, and the T polyadenylation signal from pSV2-dhfr. It includes a 44 bp polylinker. |
| References |
Cate RL, et al. Isolation of the bovine and human genes for Mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685-698, 1986. PubMed: 3754790
Cate RL, Donahoe PK. DNA sequences, recombinant DNA molecules and processes for producing mullerian inhibiting substance-like polypeptides. US Patent 5,047,336 dated Sep 10 1991
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