| Insert |
DNA: cDNA Insert lengths(kb): 0.9739999771118164 Tissue: scrapie-infected brain Gene product: prion protein (p27-30)(prion protein) [PRIP] Alleles: A1, A1, A1, A1, A2, A2, B1, B1, B2, B2, A2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 0.9; EcoRI--5.4; HindIII--5.4; BamHI--5.4; PstI--5.4. Insert includes sequence from nt 2 to nt 808, with first in-frame ATG at nt 47, encoding a protein of 254 amino acids. Hybridization conditions suggest homology between hamster and human sequences is about 80%. Hybridizes to a unique human genomic BamHI fragment of 10 kb (0.1X SSC, 68C) and human mRNA of about 3 kb. Enzyme(s) not detecting polymorphism: ApaI, ApaLI, AvaI, AvuI, BanI, BamHI, BclI, BglI, BglII, BstEII, BstNI, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, NheI, NruI, PstI, RsaI, SmaI, SstI, StuI, TaqI, XbaI, XmnI (20 unrelated European |
| References |
Harris MS, et al. A BanI RFLP at a deletion hotspot in the human dystrophin gene. Nucleic Acids Res. 18: 385, 1990. PubMed: 1970164
Wu Y, et al. A PvuII RFLP detected in the human prion protein (PrP) gene. Nucleic Acids Res. 15: 3191, 1987. PubMed: 2882483
Robakis NK, et al. Localization of a human gene homologous to the PrP gene on the p arm of chromosome 20 and detection of PrP-related antigens in normal human brain. Biochem. Biophys. Res. Commun. 140: 758-765, 1986. PubMed: 2877664
Robakis NK, et al. Isolation of a cDNA clone encoding the leader peptide of prion protein and expression of the homologous gene in various tissues. Proc. Natl. Acad. Sci. USA 83: 6377-6381, 1986. PubMed: 3529083
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