in another host, produces protein guanine nucleotide binding protein (G protein), q polypeptide heterotrimeric guanine nucleotide-binding protein alpha q subunit, G alpha q
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Gene product: guanine nucleotide binding protein (G protein), q polypeptide( heterotrimeric guanine nucleotide-binding protein alpha q subunit, G alpha q) [Gnaq]
Target Gene: guanine nucleotide binding protein (G protein), q polypeptide
Insert Size (kb)
1.200
Media
ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2; HindIII--5.2; PstI--5.2; XbaI--5.2; XhoI--5.2.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.75; BglII--0.93; EcoRI--1.02; NdeI--0.48; NotI--0.04; PstI--0.08, 1.15; PvuII--1.15; SacI--0.22; SalI--0.26, 0.97; SmaI--0.02; XbaI--0.94; XhoI--1.02.
Deposited with the understanding that the DNAs not be used in human experiments.
The protein produced is the Q209L mutation (leucine replacing glutamine at codon 209) with a hemagglutinin tag.
Nucleotides 93-95 and 687 of the GenBank sequence have been altered to add restriction sites for SalI and BamHI respectively. This does not alter the amino acid sequence.
Encodes a hemagglutinin epitope replacing nt 409-426 of the GenBank sequence.
Restriction site(s) at the 3' end of the insert for linearizing the construct: NsiI.
Wedegaertner PB, et al. Palmitoylation is required for signaling functions and membrane attachment of Gq-alpha and Gs-alpha. J. Biol. Chem. 268: 25001-25008, 1993. PubMed: 8227063
