| 產品名稱 |
pUN70 [Y302, pNN336] |
| 商品貨號 |
B226474 |
| Designations |
pUN70 [Y302, pNN336] |
| Depositors |
SJ Elledge |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 5.6999998092651370 Vector: pUN70 (phagemid) Promoters: Promoter lac Construction: pUC18 Marker(s):URA3,ampR Construct size (kb): 5.699999809265137 Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: M13+
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4 |
| Applications |
YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; KpnI--5.2; BamHI--5.2; PvuII--5.0, 0.3. YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA). |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604
|
| Related Products |
component of:ATCC 87634 component of:ATCC 87637 |
| Shipped |
freeze-dried |
