Restriction digests of the clone give the following sizes (kb): PstI--3.7, 1.8; EcoRI--5.6; HindIII--3.9, 1.0, 0.7; BamHI--5.6. Linked to X-linked hypohidrotic ectodermal dysplasia (theta = 0.02, z = 13.44). No mouse Pgk-1 pseudogene is on the X chromosome, and the mouse genome contains several more pseudogenes than does the human genome. Includes 82 bp of 5' and 437 bp of 3' untranslated sequence, the complete coding region, and a polyadenylation signal 17 bp from the poly(A) site. The PstI RFLP is impossible to detect because of an overlapping 6.0 kb autosomal fragment. The autosomal fragment has an XbaI site so genomic DNA can be digested with XbaI/PstI. This RFLP showed X-linked inheritance in 10 families. Enzyme(s) not detecting polymorphism: EcoRI, HindII, MspI, TaqI, AvaII, HindIII, SacI, BamHI, MboII, HpaI. |
