| 產品名稱 |
D-157 |
| 商品貨號 |
B224633 |
| Designations |
D-157 |
| GenBank Number |
M85168
|
| Species |
Homo sapiens, human |
| Depositors |
Y Chen |
| Vector |
Construct size (kb): 4.760000228881836 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AGL
Genomic copy number: unique
Gene name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen
debranching enzyme, glycogen storage disease type III)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p21
Tissue: muscle
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.563
Cross references: DNA Seq. Acc.: M85168 Insert lengths(kb): 1.562999963760376 Tissue: muscle Gene product: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) [AGL] |
| Insert Size (kb) |
1.563 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.0, 0.5; HindIII--3.2, 1.1, 0.4; SacI/PstI--4.7; BamHI--4.0, 0.6; BglII--4.7; XbaI--4.55, 0.25. The insert contains the following restriction sites (kb from the 5' end): BamHI--0.84; BglII--0.72; EcoRI--1.42; HindIII--0.39; NcoI--0.27, 0.36; XbaI--1.28. Corresponds to nt 954-2490 of the published sequence, containing part of the coding region. |
| References |
Yang BZ, et al. Molecular cloning and nucleotide sequence of cDNA encoding human muscle glycogen debranching enzyme. J. Biol. Chem. 267: 9294-9299, 1992. PubMed: 1374391
Yuan-Tsong Chen, personal communication
|
