Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Adult human male, Vietnam, 1966 (?)
Product Format
frozen
Storage Conditions
Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures: 2-8°C
Live Cultures: See Protocols section for handling information
Type Strain
no
Antibiotic Resistance
Chloroquine-resistant
Comments
Chloroquine-resistant
Continuous culture
Cultivation
Medium
ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37°C
Culture System:In vitro culture in human erythrocytes
Cryopreservation
Harvest and Preservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.
Aspirate supernatant using sterile Pasteur pipet.
Resuspend pellet gently in remaining supernatant.
Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
Add the remaining 4 volumes of glycerolyte and gently agitate.
Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.).
Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635
Trager W, Jensen JB. Human malaria parasites in continuous culture. Science 193: 673-675, 1976. PubMed: 781840
Jensen JB, Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J. Parasitol. 63: 883-886, 1977. PubMed: 335035
