DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Modification: dG tailed Vector end: PstI Modification: dG tailed Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: HLA-DRB* Gene name: major histocompatibility complex, class II, DR beta (GB:A06800) Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 p21.3 Tissue: cell line IBW9 (beta lymphoblasts) Type of DNA: cDNA Insert end: Modification: dC tailed Insert end: Modification: dC tailed Insert size (kb): 1.162 Cross references: DNA Seq. Acc.: A06800 Nucleotides 1-1162 of the insert correspond to nucleotides 1-1162 of A06800.
Insert lengths(kb): 1.161999940872192
Tissue: cell line IBW9 (beta lymphoblasts)
Gene product: major histocompatibility complex, class II, DR beta (GB:A06800) [HLA-DRB*]
Insert Size (kb)
1.162
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.5 and smaller fragments; SstI--5.6; StuI--5.6.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.10; ScaI--0.30; PstI--0.36, 0.49, 0.97; XmnI--0.61; HindIII--0.89.
References
Mach BF, et al. DNA sequences coding for the DR-beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto. US Patent 5,169,941 dated Dec 8 1992
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
