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pYAC4
pYAC4
規(guī)格:
貨期:
編號(hào):B223672
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pYAC4
商品貨號(hào) B223672
Designations pYAC4
Depositors MV Olson
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 11.4540004730224600
Vector: pYAC4 (YAC)
Marker(s): TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.454
Features: marker(s): HIS3
marker(s): SUP4
marker(s): TRP1
marker(s): URA3
marker(s): ampR
other: telomere
replicon: ARS1
replicon: pMB1
centromere: CEN4
Applications
YL-type (YAC) shuttle vector
Shuttle vector
Vector for constructing genomic libraries
Comments
Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments
A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825

Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741

Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791

Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163

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