| Vector |
Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(-)
Intact vector size: 3.200
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7 - EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII - SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SNF2L
Genomic copy number: unique
Gene name: SNF2 (sucrose nonfermenting, yeast, homolog)-like
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q25
Tissue: fibroblast
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 3.6
Cross references: DNA Seq. Acc.: M88163 Insert lengths(kb): 3.599999904632568 Tissue: fibroblast Gene product: SNF2 (sucrose nonfermenting, yeast, homolog)-like [SNF2L] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.6, 0.7, 0.3; HindIII--4.0, 1.4, 1.3, 0.2; PvuII--2.7 (doublet), 1.35; SacI--5.5, 0.9, 0.45; SalI--4.0, 2.2, 0.6. The insert contains the following restriction sites (bp from the 5' end): EcoRI--2681, 2938; HindIII--871, 2122, 3478; NcoI--2933; NdeI--434; PstI--998, 2006; PvuII--2603; SacI--2299, 3180; SalI--900, 1496; XhoI--2558. Contains the complete coding sequence (nt 142-3072). Sequence shows strong homology to the Saccharomyces cerevisiae transcription activator genes SNF2/SWI2 and STH1. The insert detects a 4.4 kb transcript in the following human tissues: heart, brain, placenta, lung, liver, skeletal muscle and kidney. |
