Restriction digests of the clone give the following sizes (kb): EcoRI--8.2; PstI--8.2; XbaI--8.2. Recombinant deletion YACs can be recovered by digestion with BglII, EcoRI, HindIII, PstI, SacI or XbaI, recircularization, and transformation into E. coli. pBCL8.1 should be linearized with SalI before transformation into human YAC-containing yeast strains. Lys+ Trp+ Ura- colonies should be selected. Chromosome acentric fragmentation vector containing LYS2 for use with artificial chromosomes containing Alu repeats. The order of the major features in the plasmid is: LYS2 - HindIII - telomere - SalI - Alu repeats - pMB1 ori - ampR - SacI - XbaI - PstI - EcoRI. |
