| 產品名稱 |
pWL625Amy & pBR325Amy |
| 商品貨號 |
B222100 |
| Designations |
pWL625Amy & pBR325Amy |
| Species |
Geobacillus stearothermophilus (Donk) Nazina et al. |
| Depositors |
CPC International, Inc. |
| Applications |
The Escherichia coli host contains two plasmids: pWL6225 and pBR325 (ATCC 31792). |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmlR
Cross references: DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmlR
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 5.4
Cross references: Insert lengths(kb): 5.400000095367432 Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) |
| Insert Size (kb) |
5.400 |
| Media |
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The total size of the clone is 20.4 kb in pWL625 or 11.2 kb in pBR325. The Escherichia coli host contains two plasmids: pWL6225 and pBR325 (ATCC 31792). |
| References |
Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
