| 產品名稱 |
huB29/pUC19 |
| 商品貨號 |
B221358 |
| Designations |
huB29/pUC19 |
| GenBank Number |
M80461
|
| Species |
Homo sapiens, human |
| Depositors |
B Mueller |
| Vector |
Construct size (kb): 3.910000085830689 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGB
Genomic copy number: unique
Gene name: immunoglobulin-associated beta (B29)
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23
Tissue: tonsil
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.224
Cross references: DNA Seq. Acc.: M80461 Insert lengths(kb): 1.223999977111816 Tissue: tonsil Gene product: immunoglobulin-associated beta (B29) [IGB] |
| Insert Size (kb) |
1.224 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--3.9; EcoRI--2.7, 1.2; BglI--1.45, 1.35; PstI--3.9; KpnI--3.7, 0.2. The insert contains the following restriction sites (nt from the 5' end): BglI--20, KpnI--117; NcoI--15, 1063; PvuII--81, 278, 464, 804. Contains the complete coding sequence (nt 15-702). |
| References |
Muller B, et al. Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29. Eur. J. Immunol. 22: 1621-1625, 1992. PubMed: 1534761
Brigitte Mueller, personal communication
|
