人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Crithidia oncopelti (Noguchi and Tilden) Hanson and McGhee
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
Crithidia oncopelti (Noguchi and Tilden) Hanson and McGhee
Crithidia oncopelti (Noguchi and Tilden) Hanson and McGhee
規格:
貨期:
編號:B221079
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Crithidia oncopelti (Noguchi and Tilden) Hanson and McGhee
商品貨號 B221079
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Aposymbiotic
Product Format frozen
Type Strain no
Comments
species description
Ornithine-arginine metabolism
Ultrastructure of symbiotic bacteria
Proteolytic activities
isoleucine requirement and threonine deaminase
endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification
Acetylornithinase and ornithine acetyltransferase
Ultrastructural differences between species with and without endosymbionts
Medium ATCC® Medium 355: Crithidia medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Cryopreservation

1. ? Prepare a 10% (v/v) sterile DMSO solution in fresh ATCC Medium 1034.?

2.?? Transfer a culture at peak density to centrifuge tubes and centrifuge at 525 x g for 5 minutes.

3.?? Remove the supernatant and resuspend the cells in ATCC medium 1034 to a concentration of 2 x 106 to 2 x 107 cells/ml.

4.?? Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/ml and 5% (v/v) DMSO.

5.?? Distribute the cell suspension in 0.5 ml aliquots into 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).? The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.

6.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? If the freezing unit can compensate for the heat of fusion, maintain rate at??????? -1°C/min through the heat of fusion.? At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately???????????? -1°C/min.) ?

7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer.

8.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial.

9.?? Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 1034 in a 16 x 125 mm screw-capped test tube. Incubate upright at 25°C with caps screwed on tightly.

Name of Depositor KP Chang
Chain of Custody
ATCC <<--KP Chang<<--ATCC 12982
References

Chang KP. Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti. J. Protozool. 21: 699-707, 1974. PubMed: 4217371

Figueiredo EN, et al. Enzymes of the ornithine-arginine metabolism of trypanosomatids of the genus Crithidia. J. Protozool. 25: 546-549, 1978.

Chang KP, et al. Effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149, 1978. PubMed: 660567

Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594

Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925

Cross References

Nucleotide (GenBank) : U67436 Crithidia oncopelti large subunit 24S rRNA gene, 5'end, LS1 region.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板： 欧美金妇欧美乱妇xxxx| 亚洲爱婷婷色婷婷五月| 欧美日本国产va高清cabal| 俄罗斯大荫蒂女人毛茸茸| 狠狠狠色丁香综合婷婷久久| 无码中文av波多野结衣一区| 成在线人永久免费视频播放| 成在人av抽搐高潮喷水流白浆 | 成人片黄网站a毛片免费观看| 亚洲国产桃花岛一区二区| 日韩精品一区二区三区在线观看| 久久国产劲暴∨内射新川| 被c到高潮疯狂喷水国产| 国产裸体xxxx视频| 国产激情久久久久久熟女老人av| 亚洲国产精品无码中文字满| 亚洲狠狠爱综合影院网页| 国产乱沈阳女人高潮乱叫老| 女人和拘做爰正片视频| 春药玩弄少妇高潮吼叫| 日本xxxx少妇高清hd| 中文字幕人乱码中文字幕| 一本久久a久久精品综合| 欧美孕妇xxxx做受欧美88| 999久久久国产精品| 无码人妻精品一区二区蜜桃网站| 欧美成aⅴ人高清免费| 久久精品亚洲中文字幕无码麻豆| 人妻中文字幕无码专区| 国产有奶水哺乳期无码avav| 欧美日产欧美日产国产精品| 欧洲极品少妇| 无人区码一码二码w358cc| 亚洲精品久久66国产高清| 亚洲国产成人久久综合| 久久国产精品成人影院| 日韩卡二卡三卡四卡永久入口| 国产亚洲精品成人aa片| 亚洲午夜无码久久久久| 国产成人精品久久一区二区| 欧美性猛交久久久乱大交小说|