| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.9; EcoRI--8.4, 8.4; EcoRI/HindIII--4.5, 2.6, 2.3; PstI--5.4, 4.2, 3.4, 3.0; HincII--8.4, 4.2, 3.4; consistent with deposited DNA. Codominant segregation has been demonstrated in more than 52 three-generation families. The probe appears to contain four copies of the insert. Detection of the TaqI polymorphism (alleles 10, 9, and 8 kb) was verified by CEPH. The RFLPs are detected under normal stringency (0.1X SSC). |
| References |
Bradley DG, et al. Autosomal dominant retinitis pigmentosa: exclusion of the gene from the short arm of chromosome 1 including the region surrounding the Rhesus locus. Am. J. Hum. Genet. 44: 570-576, 1989. PubMed: 2564733
Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pYNZ2) on chromosome 1p (D1S57). Nucleic Acids Res. 16: 4747, 1988. PubMed: 2454462
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