Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; HindIII--2.4; PstI--2.4; BamHI--2.4; XbaI--2.4. A general purpose plasmid vector for use in complementation analysis with plasmids having a ColE1 or pMB1 origin of replication. Constructed by M.G. Jobling. pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence. |
