| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--7.6, 3.5, 0.8; BamHI--3.4, 2.4, 2.1, 1.7, 1.6; EcoRI/HindIII--2.5, 2.2, 1.8, 1.3 (doublet), 0.9, 0.8, 0.6, 0.4, 0.3; AccI--3.6, 3.5, 1.9 (doublet); EcoRI--4.7, 3.6, 1.8, 0.9, 0.6, 0.4; consistent with deposited DNA. The structure of this plasmid is not consistent with the published description of pHHH220. The hybridization pattern of this probe with human DNA was not checked by the ATCC. Codominant segregation of the TaqI RFLP was observed in 43 three-generation families. This probe has significant lane background, but RFLPs observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI, PvuII and RsaI. |
