| 產品名稱 |
pRS421 |
| 商品貨號 |
B220100 |
| Designations |
pRS421 |
| Depositors |
JD Boeke, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine |
| Biosafety Level |
1 |
| Host |
Escherichia coli HB101 (ATCC 33694) |
| Vector Information |
Size of construct (kb): 6.233
Markers: ampR, MET15
DNA Seq. Accession number: U93716
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI XhoI ApaI KpnI
Construction: pRS401, YEp24
Host range: S. cerevisiae; E. coli
Features (with position when available):
marker(s): MET15, 421-1270
replicon: f1, 1860-2316
promoter for in vitro transcription: T7, 2486-2505
MCS: KpnI...SacI, 2513-2615
promoter for in vitro transcription: T3, 2632-2651
insert detection: lacZ', 2317-2676
promoter: lac, 2721-2749
replicon: pMB1, 3075-3075
marker(s): ampR, 3833-4693
replicon: 2 micron, 4825-6166
|
| Applications |
YE-type (episomal) shuttle vector
Vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--1.7, 4.5; XbaI--3.2, 2.0, 1.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37°C |
| References |
Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447
Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
|
| Related Products |
Component of: ATCC 87476 Component of: ATCC 87529 Component of: ATCC 87535 |
| Shipped |
frozen |
| Shipping Information |
Frozen glycerol stock of Escherichia coli containing the plasmid |
