人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Crithidia fasciculata Leger
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Crithidia fasciculata Leger
Crithidia fasciculata Leger
規(guī)格:
貨期:
編號(hào):B219994
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Crithidia fasciculata Leger
商品貨號(hào) B219994
Strain Designations Wallace
Application
assay of biopterin
assay of pteridines
catabolizes tryptophan L-tryptophan
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
mosquito, Culex pipiens, St. Paul, MN, 1942
Product Format frozen
Type Strain no
Comments
Ornithine-arginine metabolism
Trypanosomatids from fruit
Distribution of carbohydrate epitopes
Inhibitors of ergosterol biosynthesis
Riboprinting and taxonomy
Properties of dihydrofolate reductase
Monoclonal antibodies for identification
Catabolism of tryptophan
Adaptive use of N2-dimethyl-substituted pterins
cyclopropane fatty acid
Multiple distinct site-specific elements in miniexon arrays
use of mutants in detecting genetic recombination
endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification
effect of temperature and osmolarity on growth
Medium ATCC® Medium 355: Crithidia medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Cryopreservation

1. ? Prepare a 10% (v/v) sterile DMSO solution in fresh ATCC Medium 355.?

2.?? Transfer a culture at peak density to centrifuge tubes and centrifuge at 525 x g for 5 minutes.

3.?? Remove the supernatant and resuspend the cells in ATCC medium 355 to a concentration of 2 x 106 to 2 x 107 cells/ml.

4.?? Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/ml and 5% (v/v) DMSO.

5.?? Distribute the cell suspension in 0.5 ml aliquots into 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).? The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.

6.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? If the freezing unit can compensate for the heat of fusion, maintain rate at??????? -1°C/min through the heat of fusion.? At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately???????? ????-1°C/min.) ?

7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer.

8.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial.

9.?? Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 355 in a 16 x 125 mm screw-capped test tube. Incubate upright at 25°C with caps screwed on tightly.

Name of Depositor HN Guttman
Chain of Custody
ATCC <<--HN Guttman<<--F.G. Wallace
Year of Origin 1942
References

Figueiredo EN, et al. Enzymes of the ornithine-arginine metabolism of trypanosomatids of the genus Crithidia. J. Protozool. 25: 546-549, 1978.

Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989.

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421

J. Parasitol. 29: 196-205, 1943.

Rahman MD, Pascal RA Jr.. Inhibitors of ergosterol biosynthesis and growth of the trypanosomatid protozoan Crithidia fasciculata. J. Biol. Chem. 265: 4989-4996, 1990. PubMed: 2318878

Analytical microbiology. vol. 2New York: Academic Press; 1972.

Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441

Iwai K, et al. Purification and properties of dihydrofolate reductase from Crithidia fasciculata. Agric. Biol. Chem. 45: 113-120, 1981.

Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982.

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989.

Teixeira MM, et al. Characterization of the target antigens of Phytomonas-specific monoclonal antibodies. J. Eukaryot. Microbiol. 42: 232-237, 1995.

Seed JR, et al. The catabolism of Tryptophan to indole-3-ethanol by Crithidia fasciculata and Phytomonas davidi. J. Protozool. 32: 20-25, 1985.

Ziegler I, et al. Adaptive use of N2-Dimethyl-substituted pterins by cultures of Crithidia fasciculata. J. Protozool. 28: 354-357, 1981.

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515

Glassberg J, et al. Isolation and partial characterization of mutants of the trypanosomatid Crithidia fasciculata and their use in detecting genetic recombination. J. Protozool. 32: 118-125, 1985. PubMed: 3857343

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925

Da Silva JB, Roitman I. Effect of temperature and osmolarity on growth of Crithidia fasciculata, Crithidia hutneri, Crithidia thermophila, and Herpetomonas samuelpessoai. J. Eukaryot. Microbiol. 29: 269-272, 1982.

Fernandes O, et al. Mini-exon gene sequences define six groups within the genus Crithidia. J. Eukaryot. Microbiol. 44: 535-539, 1997. PubMed: 9435125

Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198

Baker H, et al. Biopterin content of human and rat fluids and tissues determined protozoologically. Am. J. Clin. Nutr. 27: 1247-1253, 1974. PubMed: 4447093

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
主站蜘蛛池模板： 国产精品国产三级国av| 国产综合一区二区三区黄页秋霞| 波多老师无码av中字专区| 99这里有精品视频视频| 国产乱子伦一区二区三区=| 亚洲人成网站在线观看69影院| 成人免费xxxxx在线观看| 在线天堂www在线国语对白| 成人精品视频在线观看不卡| av无码午夜福利一区二区三区| 亚洲精品一区久久久久久| 亚洲最新无码中文字幕久久| 国精产品一区一区三区有限在线| 曰韩欧美群交p片内射| aaa欧美色吧激情视频| 嫩草国产露脸精品国产软件| 国产成人精品一区二三区| 末发育娇小性色xxxxx视频| 国产三级不卡在线观看视频| 偷自拍亚洲视频在线观看99| 夜夜躁狠狠躁日日躁视频| 久久久99精品免费观看| 中文字幕av高清片| 国产成人无码午夜福利在线直播 | 成人爱做日本视频免费| 国产乱人伦偷精品视频aaa| 日本japanese漂亮丰满| 国产精品国产三级国产专区53| 老妇肥熟凸凹丰满刺激| 国产天美传媒性色av出轨| 国产成人亚洲精品无码青青草原| 精品无码专区毛片| 人人妻人人澡人人爽人人dvd| 日韩精品无码一区二区忘忧草 | 午夜dj在线观看高清在线视频完整版 | 亚洲天天做日日做天天谢日日欢| 色婷婷综合久久久久中文字幕| 日韩精品无码免费一区二区三区| 精品一区二区成人精品| 国产人妻777人伦精品hd| 国产成a人亚洲精品无码樱花|