| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4 (doublet), 8.0, 5.4, 4.2, 3.6, 3.4, 1.6; BamHI--22.0, 8.6, 6.2, 3.7, 3.2, 2.7; HindIII--22.0, 10.0, 8.4, 3.8; BglI--15.0, 7.2, 6.4 (doublet), 3.2 (doublet), 1.4, 1.2; SalI--22.0 (doublet), 2.7. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
