Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 0.85; BglI--1.6, 1.25, 0.95, 0.68, 0.45, 0.35, 0.3; KpnI--5.1, 0.5; PstI--5.0, 0.45, 0.1; SacI--5.0, 0.9. The insert contains the following restriction sites (bp from the 5' end): BglI--212, 519; KpnI--470; PstI--83, 202, 623; SacI--64; SmaI--761. BZRP has been localized to 22q13.31 on the human chromosome. a human genomic EcoRI fragment of approximately 7 kb, and a human genomic HindIII fragment of 6.5 kb. The hamster genomic EcoRI fragment is larger. Contains the complete coding sequence which is identical to that reported in GenBank accession M36035. This insert also has a longer 5' untranslated region. Detects RNA of approximately 1 kb in Northern blots, The insert was cloned into a shuttle vector that regulates expression of inserts by the cytomegalovirus (CMV) major early immediate promoter and contains the human growth hormone (GH) terminator. |
