Restriction digests of the clone give the following sizes (kb): AccI--4.8; BamHI--4.8; EcoRI--3.0, 1.75; HindIII--4.8; PvuII--2.4, 1.0, 0.85, 0.48. Evidence of linkage (theta = 0.00, LOD score = 7.55) was found between the skeletal muscle glycogen phosphorylase (PYGM) gene on 11q and multiple endocrine neoplasia type 1 (MEN1). Polymorphism also detected with TaqI. Hybridization solution contained about 100 ug/ml total human DNA and 50% formamide. Subcloned from cosmid cMCMP1 isolated by using the HM11 3' myophosphorylase cDNA. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, RsaI. |
