Aspergillus niger var. macrosporus Koaze, anamorph
Depositors
OmniGene, Inc., RR Yocum, OmniGene, Inc.
Applications
produces protein glucoamylase
Vector
Construct size (kb): 8.30
DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGM30 Intact vector size: 8.800 Type of vector: plasmid Vector end: XhoI Modification: BssHII adaptor Vector end: XbaI Cloning sites: XbaI XhoI Polylinker sites: Construction: pRY271, pGM11 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): URA3 promoter: TPI leader peptide: DEX4 coding sequence: DEX4 transcription terminator: TPI replicon: pMB1 marker(s): ampR replicon: 2 micron Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT: Genome: Aspergillus niger var. macrosporus Gene name: glucoamylase Contains complete coding sequence?: Y Type of DNA: cDNA Insert 5' end: BssHII Insert 3' end: XbaI Insert size (kb): 2.2 Cross references:
Insert lengths(kb): 2.20
Gene product: glucoamylase
Target Gene: glucoamylase
Insert Size (kb)
2.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): XbaI/XhoI--6.6, 2.1; SmaI--8.6; XbaI--8.6.
When transformed into yeast strain RD61-1D, pRD112 yields approximately 100 units of glucoamylase activity.
References
Maine GT, et al. Expression and secretion vector for yeast containing a glucoamylase signal sequence. US Patent 5,024,941 dated Jun 18 1991
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
