-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
HCC1143 is a poorly differentiated cell line that was initiated on August 30, 1994; it took 29 months to establish. HCC1143 was derived from an invasive ductal carcinoma with no lymph node metastases.
The cells are negative for expression of Her2-neu but positive for expression of p53. HCC1143 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for? expression of estrogen receptor (ER) and progesterone receptor (PR). DNA isolated from an EBV-transformed lymphoblastoid cell line, HCC1143 BL, from the same patient is available as ATCC
?CRL-2362D.
Quality Control Specifications
DNA Quality Control Information:
· > 100 kb in size by agarose gel electrophoresis
· No RNA detected in the agarose gel
· A260/A280 ratio 3 1.8
· Tested and verified for PCR amplification and restriction digestion.
