| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.239, 0.619, 0.708, 4.056, 2.239, 3.848, 4.116, 6.114, 0.431, 3.240, 1.725, 1.267, 0.937, 0.790, 0.485, 2.085, 1.906, 1.533, 0.429, 1.983, 1.295. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.88, 1.3, 1.5, 1.7, 1.9, 2.0, 2.2, 3.2, 3.8, 4.0, 6.3. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70206, 70747, 70868, 70888. |
