| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.3; SacI--7.0, 3.0, 0.9. Shows transforming activity; insert contains 3 SacI sites (major fragments 3.0, 2.7, 0.85 kb), as well as XhoI, PvuII, XbaI, KpnI, SphI, ClaI, BglII sites; plasmid cut with SacI gives 7.0, 3.0, 0.85 kb fragments. This probe contains the transforming form of the HRAS1 gene mutated by a G to T transversion at the 12th codon; it also contains 3' and 5' flanking sequences. |
| References |
Iwahana H, et al. A new RFLP in intron 1 of the human c-Ha-ras1 gene and its close relationship with the variable tandem repeats in the region 3' to the gene. Oncogene 5: 1049-1053, 1990. PubMed: 1973828
Pulciani S, et al. Oncogenes in human tumor cell lines: Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc. Natl. Acad. Sci. USA 79: 2845-2849, 1982. PubMed: 6953433
Santos E, et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298: 343-347, 1982. PubMed: 6283384
Reddy EP. Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene. Science 220: 1061-1063, 1983. PubMed: 6844927
Pulciani S, et al. Transforming genes in human tumors. J. Cell. Biochem. 20: 51-61, 1982. PubMed: 6761348
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