Restriction digests of the clone give the following sizes (kb): BamHI/SalI--17, 9.4, 7.6, 6.5, 3.0. Insert includes 3 Alu sequence elements and some mouse repetitive sequences. A unique probe can be generated from a 0.92 kb EcoRI fragment. The tk-C sequences were isolated from a phage genomic library made from DNA of a transfected mouse cell carrying the human tk-C gene. |
