Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.5, 9.5; EcoRI--25.0, 13.5, 9.5; SalI--20.0, 9.5, 9.2, 4.2, 3.9. Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in. The polylinker sites given are those associated with the longer arm. The sites associated with the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI NcoI XbaI. The restriction sites to be used for conversion to phagemid are: ClaI BspMII SalI. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The order of the major features in this plasmid is: arm - ClaI BspMII SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/SalI - stuffer - SalI/MCS/XbaI - ClaI SalI - arm. |
