| Vector Information |
Size (kb): 6.2199997901916500 DESCRIPTION OF VECTOR:
Intact vector size: 6.220
Type of vector: plasmid
Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI
Construction: pRS314
Features (with orientation and position when available):
replicon: ColE1, 1-362
coding sequence: bla, <-, 934-1794
replicon: ARS4, 2066-2442
centromere: CEN6, 2028-2053
marker(s): TRP1, ->, 2976-3650
promoter: PADH, 4437-5165
MCS: NdeI..PstI, ->, 5661-5730
ribosome-binding site: GAL4, ->, 5166-5607
transcription terminator: ADC1 transcriptional terminator (TADH), 5748-5938 Vector: pCD.2 (plasmid) Promoters: Promoter PADH Construction: pRS314 Marker(s):crbR Construct size (kb): 6.21999979019165 Features: marker(s): TRP1
promoter: PADH
replicon: ARS4
replicon: ColE1
MCS: NdeI..PstI
ribosome-binding site: GAL4
transcription terminator: ADC1 transcriptional terminator (TADH)
centromere: CEN6
coding sequence: bla |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; PstI--6.2; HindIII--4.5, 1.7; NdeI--6.2. Useful in two hybrid screening in yeast. A CEN-based plasmid with the Gal4' DNA binding domain. It has lower copy number than 2 micron based plasmids. Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5' GATC overhangs in all three reading frames. |
