Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (approximate kb): BamHI- 0.24- PstI- 0.45- BamHI- 1.0- BamHI- 0.63- XhoI- 0.29- PstI- 2.3- XbaI- 1.0- BamHI- 0.6- XbaI- 1.1- XbaI- 2.9- BamHI- 0.25- BamHI.
Carries pG59/T1 with the wild-type gene
The insert includes a continuous open reading frame of 2682 nt encoding a protein of 894 amino acids and a molecular weight of 102 kDa.
The probe, pG59/T1, has been deposited in Escherichia coli RR1 as ATCC 37668 and in Saccharomyces cerevisiae C151/L1 as ATCC 66312.
Tzagoloff A, et al. Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli. J. Biol. Chem. 263: 850-856, 1988. PubMed: 2826465
