| 產品名稱 |
HFBDV22 |
| 商品貨號 |
B216862 |
| Designations |
HFBDV22 |
| GenBank Number |
T06562
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TLE3*
Gene name: transducin-like enhancer protein (enhancer of split)
Contains complete coding sequence?: N
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M99438; EST: EST04451
Nucleotides 1-335 of the insert correspond to
nucleotides 2007-2341 of M99438 with 97% identity.
DNA Seq. Acc.: T06562; EST: EST04451; Primer: M13-21; THC
id: THC117710
DNA Seq. Acc.: T06563; EST: EST04452; Primer: M13 Reverse Insert lengths(kb): 0.800000011920929 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: transducin-like enhancer protein (enhancer of split)( transducin-like enhancer of split 3, transducin-like enhancer 3) [TLE3*] |
| Insert Size (kb) |
0.800 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.0; EcoRI--3.2, 0.8. Verified to contain EST04451 by single-pass sequencing. Based on the size of the insert and the alignment with GenBank accession M99438 (2357 nt, CDS 22-2340), this clone appears to contain part of the coding region and the 3' untranslated region. EST04452 does not match a nucleotide sequence record. |
| References |
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
|
