| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--15.0, 9.6, 7.4, 5.8, 3.5, 2.6; EcoRI--10.8, 7.4, 5.6 (doublet), 5.2, 3.5, 2.9, 1.2, 0.82; NotI-->23, 7.4, 4.2; SalI-->23, 2.4; HindIII--6.2, 5.6 (doublet), 4.6, 3.8, 3.2, 3.1, 2.6, 1.4, 1.2, 1.0, 0.9, 0.58, 0.39, 0.27; BglII--8.8 (doublet), 6.6, 5.2, 3.2, 2.6 (doublet), 2.0, 1.4. Codominant segregation of RFLP was observed in 35 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs observed after competitive hybridization with total human DNA. Enzyme(s) not detecting polymorphism: PstI. |
| References |
White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325
Bragg T, et al. Isolation and mapping of a polymorhphic DNA sequence (cTB14.15) on chromosome 10 (D10S32). Nucleic Acids Res. 16: 11392, 1988. PubMed: 2905056
Yusuke Nakamura, personal communication
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