| 產品名稱 |
pGAR1 [CMCC 1775] |
| 商品貨號 |
B213750 |
| Designations |
pGAR1 [CMCC 1775] |
| Species |
Aspergillus awamori Nakazawa |
| Depositors |
Cetus Corp., A Belt, Cetus Corp. |
| Vector |
Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACYC184
Intact vector size: 4.244
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI HindIII BamHI SalI
Polylinker sites:
Construction: p15A, pSC101, Tn9
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): cmlR, tetR
replicon: p15A
Cross references: DNA Seq. Acc.: X06403 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Aspergillus awamori
Gene name: glucoamylase
Contains complete coding sequence?: U
Strain: NRRL 15271
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 3.4
Cross references: Insert lengths(kb): 3.400000095367432 Gene product: glucoamylase |
| Insert Size (kb) |
3.400 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.4 (intron 1); BglII--1.0 (intron 3); PstI--1.1, 3.0 (exon 4, 3' flanking); BamHI--1.9 (exon 5); SalI--2.6 (3' flanking). Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.4; BamHI--4.1, 3.1; PstI--3.8, 1.8 (doublet). The insert detects a mRNA of 2.2 kb. |
| References |
Nunberg J, et al. Glucoamylase cDNA. US Patent 4,794,175 dated Dec 27 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
