Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BglII--4.8; HindIII--3.0, 1.6, 0.2; NotI--4.8; NotI/EcoRI--3.0, 1.8. The insert includes the following restriction sites (nt positions according to the published sequence): HindIII--9, 244; PstI--736; SacI--822; BamHI--851; BglII--1116. The insert extends from the 5' end of the published sequence to the EcoRI site at nt 1786, encoding the signal peptide and the amino terminal portion of the extracellular domain. The open reading frame extends from nt 207 to nt 7247. There is approximately 1 kb of 3' untranslated sequence which has not been fully characterized. Subcloned from lambdazN7. |
