| 產品名稱 |
Adeno-associated virus 4 |
| 商品貨號 |
B213432 |
| Classification |
Parvoviridae, Dependovirus, Adeno-associated virus-4 |
| Deposited As |
Adeno-associated virus type 4 |
| Agent |
Adeno-associated virus 4 |
| Strain |
none |
| Positive for Mycoplasma |
Yes |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Monkey cells infected with SV-15 virus |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Depends upon adenovirus for replication. *Helper virus may be inactivated at 56C for 30 min. |
| Effect on Host |
Cytopathic effect: AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing. AAV produces no CPE.? Helper adenovirus induces refractile rounding and cell sloughing.? Non-cytopathic without helper virus |
| Recommended Host |
monkey kidney cells; suckling mouse Recommended Host:? LLC-MK2 (ATCC? CCL-7.1TM) |
| Growth Conditions |
Temperature: 37.0°C Duration: Evidence of virus growth can be expected within 2-3 days. For best results cells should be 24-48 hours old and 80%-90% confluent (not 100%) at the time of infection. |
| Name of Depositor |
WP Parks |
| Source |
Monkey cells infected with SV-15 virus |
| References |
Chiorini JA, et al. Cloning of adeno-associated virus type 4 (AAV4) and generation of recombinant AAV4 particles. J. Virol. 71: 6823-6833, 1997. PubMed: 9261407
Parks WP, et al. Physical assay and growth cycle studies of a defective adeno-satellite. J. Virol. 1: 171-180, 1967. PubMed: 4990036
|
| Cross References |
Nucleotide (GenBank) :
U89790
Adeno-associated virus 4, complete genome.
Nucleotide (GenBank) :
NC_001829
Adeno-associated virus 4, complete genome.
|
