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pHDR5A
pHDR5A
規格:
貨期:
編號:B212702
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pHDR5A
商品貨號 B212702
Designations pHDR5A
GenBank Number

M14758

Species Homo sapiens, human
Depositors MM Gottesman
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.379999995231628
Tissue: carcinoma KB-C2.5 cell line
Gene product: P glycoprotein 1/multiple drug resistance 1 [MDR1]
Insert Size (kb) 1.380
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
The insert contains the following restriction sites (at nucleotide positions according to the published sequence): PvuII--1314, XmnI--1560, PvuII--1921, HindIII--2041; StuI--2168; XmnI--2292.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; HindIII--3.3, 0.9; BamHI--4.3; SacI--4.3.
The insert includes nucleotides 1178 to 2561 of the cDNA.
This probe detects a mRNA of approximately 4.4-4.5 kb.
The insert is oriented such that transcription from the T7 promoter gives the sense strand.
References

Galski H, et al. Expression of a human multidrug resistance cDNA (MDR1) in the bone marrow of transgenic mice: resistance to daunomycin-induced leukopenia. Mol. Cell. Biol. 9: 4357-4363, 1989. PubMed: 2573831

Chen CJ, et al. Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell 47: 381-389, 1986. PubMed: 2876781

Ueda K, et al. The human multidrug resistance (mdr1) gene: cDNA cloning and transcription initiation. J. Biol. Chem. 262: 505-508, 1987. PubMed: 3027054

Michael M Gottesman, personal communication

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