Genomic DNA from Yersinia pestis strain Kim [ATCC® BAA-1611™**]
Isolation
**source culture ATCC BAA-1611 is not available for distribution.
The presence of pMT1 and the absence of pCD1 and pPCP1 in BAA-1611D-5 have been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen Total DNA: At least 5 μg in 1X TE buffer OD260/OD280: 1.6 to 2.0
Shipping Information
Ship on dry ice
Preceptrol®
no
Type Strain
no
Comments
This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
**source culture ATCC® BAA-1611™ is not available for distribution.
The presence of pMT1 and the absence of pCD1 and pPCP1 in BAA-1611D-5 have been confirmed by PCR amplification of plasmid-specific sequences from extracted DNA.
Chu MC, Dong XQ, Zhou X, Garon CF. A cryptic 19-kilobase plasmid associated with U.S. isolates of Yersinia pestis: a dimer of the 9.5-kilobase plasmid. Am. J. Trop. Med. Hyg. 59(5): 679-686, 1998. PubMed: 9840581
Hare JM, McDonough KA. "High-Frequency RecA-Dependent and -Independent Mechanisms of Congo Red Binding Mutations in Yersinia pestis". J. Bacteriol. 181: 4896-4904, 1999. PubMed: 10438760
Parkhill J, et al. Genome sequence of Yersinia pestis, the causative agent of plague. Nature. 413(6855): 523-527, 2001. PubMed: 11586360
