| Insert |
DNA: cDNA Insert lengths(kb): 2.900000095367432 Tissue: umbilical vein endothelium cell line Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) [VWF] Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.2, 3.0; BamHI--7.2; EcoRI--7.2; PstI--4.2, 1.2, 0.75, 0.33; HindIII--7.2. Probes I (ATCC 59782/59783), II (ATCC 59784/59785), III (ATCC 59786/59787) and IV (ATCC 59788/59789) constitute non-overlapping fragments of the human von Willebrand factor cDNA sequence that together span essentially the entire 9 kb cDNA. Insert is a 2.9 kb EcoRI (linker)/BamHI fragment derived from lambdaHvWF6 containing about 210 bp 5' noncoding sequence and encoding a 22 residue signal peptide, 741 residues of vWAgII and the amino terminal 143 amino acids of the mature vWF subunit. |
| References |
Shelton-Inloes BB, et al. Evolution of human von Willebrand Factor: cDNA sequence polymorphisms, repeated domains, and relationship to von Willebrand Antigen II. Biochem. Biophys. Res. Commun. 144: 657-665, 1987. PubMed: 3495266
Shelton-Inloes BB, et al. Gene deletions correlate with the development of alloantibodies in von Willebrand disease. J. Clin. Invest. 79: 1459-1465, 1987. PubMed: 3033024
J E Sadler, personal communication
|
