| Insert |
DNA: genomic Insert lengths(kb): 3.599999904632568 Gene product: renin [REN] Alleles: H1, H2, J1, B1, B2, F1, F2, G1, K1, K2, G2, I1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI--3.6, 2.7; HindIII--5.9, 0.7; BamHI--2.8, 2.15, 1.3; PstI--4.0, 1.3, 1.2. This insert includes exons 2 - 5. Human renin cDNA cross-hybridizes with calf chymosin mRNA. BglI, HindIII, BglII, TaqI, RsaI, and BstXI RFLPs are detected with a mix of the inserts from pHRnX0.8 (ATCC 57174), pHRnX3.6 (ATCC 57178), and pHRnES1.9 (ATCC 57176), apparently from base changes not within the coding regions of the gene. Hybridization at reduced stringency results in cross-reaction with other members of the aspartyl protease gene family including cathepsin D (mapping to 11p15). Enzyme(s) not detecting polymorphism: EcoRI, BanI, MlnI, BstEII, HphI, MspI, SstI, DraI, PvuII, MboII, ApaI, SinI, NciI, ScaI, XbaI, HinfI, NdeI, HincII. XmnI and PstI give uninterpretable results. Tested on a small sample. |
| References |
Naftilan AJ, et al. A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. Hypertension 14: 614-618, 1989. PubMed: 2573574
Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294
Hobart PM, et al. Human renin gene: Structure and sequence analysis. Proc. Natl. Acad. Sci. USA 81: 5026-5030, 1984. PubMed: 6089171
McGill JR, et al. Chromosome localization of human renin gene (REN) by in situ hybridization. Cytogenet. Cell Genet. 45: 55-57, 1987. PubMed: 3297510
John M Chirgwin, personal communication
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