| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.8, 7.4, 6.2, 4.2, 3.9, 3.5, 2.9, 1.95; BamHI/HindIII--8.2, 6.0, 5.0, 4.6, 4.1, 3.8, 2.8, 2.4; BamHI--17.0, 4.6, 3.2, 2.7, 2.7, 1.8 + smaller; HindIII--8.0, 6.6, 6.0, 5.2, 3.8, 3.8; EcoRI--8.0, 6.1, 4.8, 3.8, 3.5, 3.5, 2.5. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Codominant segregation has been demonstrated in 50 3-generation families. |
| References |
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cKKA39) on chromosome 14 (D14S23). Nucleic Acids Res. 16: 3120, 1988. PubMed: 2897113
Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220
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