Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Protocol: It is possible to distinguish the aro1B, 1C, 1D, and aro2 mutations from the trp1, 3, 4, and 5 mutations in a doubly marked strain by the following test: The aro1B, 1C, 1D, and aro2 mutants do not grow on trp- media but will grow on trp- media with 20 mg of anthranylic acid per liter of medium. Trp- strains (except trp2) will not grow on anthranylate.
Subcultivation
Protocol: It is possible to distinguish the aro1B, 1C, 1D, and aro2 mutations from the trp1, 3, 4, and 5 mutations in a doubly marked strain by the following test: The aro1B, 1C, 1D, and aro2 mutants do not grow on trp- media but will grow on trp- media with 20 mg of anthranylic acid per liter of medium. Trp- strains (except trp2) will not grow on anthranylate.
